A simple, precise, accurate and reproducible spectrophotometric method has been developed for simultaneous estimation of Diclofenac sodium and Chlorzoxazone by employing first order derivative zero crossing method in 0.1 N NaOH. The first order derivative absorption at 288 nm (zero cross point of Chlorzoxazone) was used for quantification of Diclofenac sodium and 273.6 nm (zero cross point of Diclofenac sodium) for quantification of Chlorzoxazone. The linearity was established over the concentration range of 1.2-3.6 µg/ml and 12-36 µg/ml for Diclofenac sodium and Chlorzoxazone with correlation coefficient r 2 0.9993 and 0.9977, respectively. The mean % recoveries were found to be in the range of 98.88–102% and 99.11–101% for Diclofenac sodium and Chlorzoxazone respectively. The proposed method has been validated as per ICH guidelines and successfully applied to the estimation of Diclofenac sodium and Chlorzoxazone in their synthetic mixture.
Loading....